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INDIAN INSTITUTE OF TECHNOLOGY ROORKEE
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Matrix-Assisted Laser Desorption-Ionization (MALDI)
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About Instrument and its applications-
Mass Spectrometry (MS) is the study of systems causing the formation of gaseous ions, with or without fragmentation, which are then characterised by their mass to charge ratios (m/z) and relative abundances. Matrix-Assisted Laser Desorption-Ionization (MALDI) mass spectrometry used as a powerful, highly versatile technology with a diverse range of applications. MALDI coupled to time-of-flight mass spectrometry (MALDI-TOF MS) is used to sequence proteins, map biomolecules in tissues, identify microorganisms, and analyze several thousand biochemical assays. The sample for analysis by MALDI-TOF MS is prepared by mixing or coating with a solution of an energy-absorbent matrix which entraps and co-crystallizes the sample when dried. The matrix is ionized with a laser beam, and transfers the charge to the analytes, generating singly charged ions from analytes in the sample that are then accelerated. Ions are separated from each other on the basis of their mass-to-charge ratio (m/z) before being detected and measured using the TOF mass analyzer. The high throughput and speed associated with MALDI-TOF MS has made this technology an important tool for large-scale proteomics, imaging studies, and other analyses.

General Information-
Make- Bruker
Model- ultrafleXtreme
Year of Installation- 2024

Specifications-
1) Resolution: up to 40,000
2) Mass Accuracy: 1.5 ppm
3) Source: The IR-laser based self-cleaning ion source
4) Laser: Smartbeam II
5) Laser frequency [Hz]: 2,000 (MS) / 1,000 (TOF/TOF)
6) Mass Range: up to 1,000,000

Sample Requirements-
Powder: 5mg
Liquid: 50┬Ál

Contact Person-
Dr. Jay Singh Meena
Technical Officer-II
Tel: +91-1332-285779/286170
E-mail: jsmeena.mic2018@iitr.ac.in