Research Interests
Structural Biology, Protein Engineering, Biological NMR Spectroscopy, Algal Biotechnology, Bioremediation & Biofuels, Bio-Nanotechnology
BioSketch
Professor
10 Oct 2023 - Present
Indian Institute of Technology Roorkee
Associate Professor
22 Dec 2018 - 09 Oct 2023
Indian Institute of Technology Roorkee
Assistant Professor
19 Aug 2013 - 21 Dec 2018
Indian Institute of Technology Roorkee
Research Scientist
01 Jan 2012 - 31 Aug 2013
University of Texas Medical Branch
Postdoctoral Research Associate
01 Feb 2009 - 31 Dec 2011
Rutgers University
Visiting Fellow
10 Nov 2008 - 31 Jan 2009
Tata Institute of Fundamental Research
Visiting Scholar
01 Mar 2007 - 30 Jun 2007
European Molecular Biology Laboratory
Research
Biomolecular NMR Spectroscopy & Bio-nanotechnology
Center for Biomedical Research, SGPGI Campus, Lucknow
Protein Biochemistry & Bio-Nanotechnology
Indian Institute of Technology Jodhpur
Biomarkers & Metabolomics
L V Prasad Eye Research Institute
Structural & Computational Biology
Indian Institute of Technology Mandi
Biomolecular interactions of organometallic Compounds
North easteren hill university
Representative SCI Publications:
Structural & Computational Biology
- Tyagi JL, Sharma M, Gulati K, Kairamkonda M, Kumar D, Poluri KM* “Histidine network regulates the structure-stability features of T7 endolysin native and partially folded conformations” J Mol Liq. 2023 [In Press]
- Tyagi JL, Sharma M, Gulati K, Kairamkonda M, Kumar D, Poluri KM* “Engineering of a T7 bacteriophage endolysin variant with enhanced amidase activity” ACS Biochemistry, 2022. [In Press]
- Kairamkonda M, Sharma M, Gupta P, Poluri KM* “Overexpression of bacteriophage T4 and T7 endolysins differentially regulate the metabolic fingerprint of host Escherichia coli”, Int. J. Biol. Macmol., 221, 212-223, 2022
- Agarwal N, Jaiswal N, Gulati K, Gangele K, Nagar N, Kumar D, Poluri KM* “Molecular insights into conformational heterogeneity and enhanced structural integrity of Helicobacter pylori DNA binding protein Hup at low pH” ACS Biochemistry, 60 (43), 3236-52, 2021.
- Joshi N, Tripathi DK, Nagar N, Poluri KM* “Hydroxyl Groups on Annular ring B Dictates the Affinities of Flavonol-CCL2 Chemokine Binding interactions”, ACS Omega 6(15), 10306-10317, 2021
- Gulati K, Sarkar S, Poluri KM* “Metabolomics analysis of complex biological specimens using nuclear magnetic resonance spectroscopy”, Neuromethods (Metabolomics), 159, 155-171, 2021.
- Joshi N, Kumar D, Poluri KM* “Elucidating the molecular interactions of chemokine CCL2 orthologs with flavonoid Baicalin”, ACS Omega 5(35), 22637-22651, 2020.
- Gangele K, Gulati K#, Joshi N#, Kumar D, Poluri KM* “Molecular insights into the differential structure-dynamics-stability features of interleukin-8 orthologs: implications to functional specificity”, Int. J. Biol. Macmol., 164, 3221-3234, 2020. [#Authors contributed equally]
- Joshi N, Nagar N#, Gulati K#, Gangele K, Mishra M, Kumar D, Poluri KM* “Dissecting the differential structural and dynamics features of CCL2 chemokine orthologs”, Int. J. Biol. Macmol., 156, 239-251, 2020. [#Authors contributed equally]
- Tripathi DK, and Poluri KM* “Molecular insights into kinase mediated signaling pathways of chemokines and their cognate G protein coupled receptors”, Front Biosci (Landmark Ed), 25, 1361-1385, 2020.
- Sharma M, Tyagi JL, Poluri KM* “Quantifying bacterial cell lysis using GFP based fluorimetric assay”, Int. J. Biol. Macmol., 138, 881-889, 2019.
- Gangele K, Jamsandekar M, Mishra A and Poluri KM* “Unraveling the evolutionary origin of ELR motif using fish CXC chemokine CXCL8”, Fish Shellfish Immunol. 93, 17-27, 2019.
- Sharma M, Jaiswal N, Kumar D, Poluri KM* “Enhanced dynamics of conformationally heterogeneous T7 bacteriophage lysozyme native state attenuates its stability and activity”, Biochem. Journal 476(3), 613-628, 2019
- Gulati K, Gangele K, Kumar D, Poluri KM* “An inter-switch between hydrophobic and charged amino acids generated druggable small molecule binding pocket in chemokine paralog CXCL3” Arch. Biochem. Biophys., 662, 121-128, 2019.
- Sarkar S, Gulati K, Kairamkonda M, Mishra A, Poluri KM* “Elucidating protein-protein interactions through computational approaches and designing small molecule inhibitors against them for various diseases”, Curr Top Med Chem. 18(20), 1719-36, 2018.
- Sharma M, Kumar D, Poluri KM* “Unraveling the differential structural stability and dynamics features of T7 endolysin partially folded conformations”, BBA Gen Subjects 1862(4), 924-935, 2018
- Gulati K, Gangele K, Agarwal N, Jamsandekar M, Kumar D, Poluri KM* “Molecular cloning and biophysical Characterization of CXCL3 chemokine”, Int. J. Biol. Macmol., 107, 575-584, 2018.
- Gulati K, Jamsandekar M, Poluri KM* “Mechanistic insights into molecular evolution of species specific differential glycosaminoglycan binding surfaces in growth-related oncogene chemokines”, R Soc. Open Sci. 4(9):171059, 2017.
- Gulati K and Poluri KM* “An overview of computational and experimental methods for designing novel proteins”, Recent Pat Biotechnol. 10(3), 235-63, 2016. (Editor Choice Article).
- Gangele K and Poluri KM* “Imidazole derivatives differentially destabilize the low pH conformation of lysozyme through weak electrostatic interactions”, RSC Advances 6, 101395-403, 2016.
- Sharma M, Kumar D Poluri KM* “Elucidating the pH-dependent structural transition of T7 bacteriophage endolysin”, ACS Biochemistry 55, 4614-25, 2016.
- Gulati K and Poluri KM* “Deciphering the in vitro homo and hetero oligomerization characteristics of CXCL1/CXCL2 chemokines”, RSC Advances 6, 28213-18, 2016.
- Gulati K and Poluri KM* “Mechanistic and therapeutic overview of glycosaminoglycans: the unsung heroes of biomolecular signaling”, Glycoconjugate Journal 33(1), 1-17, 2016.
Bio-Nanotechnology & Antimicrobial agents
- Gupta P, Gupta H, Kairamkonda M, Kumar N, Poluri KM* “Elucidating the lactic acid tolerance mechanism in vaginal clinical isolates of Candida glabrata”, Med Mycol., 60, myac042, 1-11, 2022
- Tripathi S, Behera T, Poluri KM* “Biochemical insights into cadmium detoxification mechanism of Coccomyxa sp. IITRSTKM4” J Environ Chem Eng., 10, 108102, 2022
- Gupta H, Gupta P, Kairamkonda M, Poluri KM* “Molecular investigations on Candida glabrata clinical isolates for pharmacological targeting”, RSC Advances, 12(27), 17570-17584, 2022
- Kumar D, Gautam A, Tripathi DK, Poluri KM, Kundu PP, “Synthesis, characterization and biological influences of rifaximin loaded melanin/zinc oxide nanoparticles” J Drug Deliv Sci Technol., 77, 103875, 2022
- Gupta P, Poluri KM* “Elucidating the eradication mechanism of perillyl alcohol against Candida glabrata biofilms: Insights into the synergistic effect with azole drugs”, ACS Bio & Med Chem Au., 2022 [In Press]
- Gupta P, Mishra P, Prasad R, Poluri KM* “Eugenol-acacia gun-based bifunctional nanofibers as potent antifungal transdermal substitute”, Nanomedicine, 16(25), 2269-2289, 2021.
- Mishra P, Gupta P, Poluri KM, Prasad R “Eucalyptol/b-cyclodextrin inclusion complex loaded gellan/PVA nanofibers as antifungal drug delivery system”, Int. J. Pharm. 609, 121163, 2021.
- Meher MK, and Poluri KM* “Anticoagulation and antibacterial properties of heparinized nanosilver with different morphologies” Carbohydrate Polymers, 266, 118124, 2021
- Gupta P, Gupta H, Poluri KM* “Geraniol eradicates Candida glabrata biofilm by targeting multiple cellular pathways”, Appl Microbiol Biotechnol., 105, 5589-5605, 2021
- Gupta P*, Pruthi V, Poluri KM* “Mechanistic Insights into Candida biofilm eradication potential of eucalyptol”, J Appl Microbiol., 131, 105-123, 2021
- Gupta P*, Mishra P, Prasad R, Poluri KM* “Dissecting the therapeutic potency of antimicrobial peptides against microbial biofilms”, Curr Protein Pept Sci. 22(4), 336-360, 2021.
- Gupta P, Goel A, Singh KR, Meher MK, Gulati K, Poluri KM* “Dissecting the anti-biofilm potency of kappa-carrageenan capped silver nanoparticles against Candida species”, Int. J. Biol. Macmol., 172 20-30, 2021
- Sarkar S#, Gulati K#, Poluri KM* “Protein nanocomposites: Special inferences to lysozyme based nanomaterials”, Int. J. Biol. Macmol., 151, 467-482, 2020. [#Authors contributed equally]
- Pranjali P#, Meher MK#, Raj R, Prasad N, Poluri KM*, Kumar D*, Guleria A*, “Physicochemical and antibacterial properties of PEGylated zinc oxide nanoparticles dispersed in peritoneal dialysis fluid”, ACS Omega 4(21), 19255-19264, 2019. (#Authors contributed equally)
- Kumari P, Arora N, Chatrath A, Gangwar R, Pruthi V, Poluri KM*, Prasard R* “Delineating the Biofilm inhibition mechanisms of phenolic and aldehydic terpenes against Cryptococcus neoformans”, ACS Omega 4(18), 17634-17648, 2019.
- Goel A, Meher MK, Gupta P, Gulati K, Pruthi V, Poluri KM* “Microwave assisted k-carrageenan capped silver nanocomposites for eradication of bacterial biofilms” Carbohydrate Polymers, 206, 854-862, 2019.
- Guleria A, Pranjali P#, Meher MK#, Chaturvedi A#, Chakraborti S, Raj R, Prasad N, Poluri KM, Kumar D “Effect of Polyol Chain Length on Proton Relaxivity of Gadolinium Oxide Nanoparticles for Enhanced Magnetic Resonance Imaging Contrast”, ACS J.Phys.Chem C. 123, 18061-18070, 2019.
- Gupta P#, Sharma M#, Arora N, Pruthi V, Poluri KM* “Chemistry and Biology of Farnesol and its Derivatives: Quorum Sensing Molecules with Immense Therapeutic Potential”, Curr Top Med Chem. 18(22), 1937-54, 2018. (#Equal Contribution)
- Gupta P, Gupta S, Sharma M, Kumar N, Pruthi V*, Poluri KM* “Effectiveness of phyto-active molecules on transcriptional expression, biofilm matrix and cell wall components of C. glabrata and its clinical isolates”, ACS Omega 3(9), 12201-14, 2018.
- Guleria A*, Meher MK, Prasad N, Poluri KM*, Kumar D* “Physicochemical transformations of ZnO nanoparticles dispersed in peritoneal dialysis fluid: Insights into nano-bio interface interactions”, ACS J.Phys.Chem C. 121, 18598-18607, 2017.
- Gulati K#, Meher MK#, Poluri KM* “Glycosaminoglycan based resorbable polymer composites in tissue refurbishment”, Regen Med. 12(4):431-457, 2017. (#Equal Contribution)
Bio-Remediation, By-products & Biofuels
- Tripathi S, Kairamkonda M, Gupta P, Poluri KM* “Dissecting the molecular mechanisms of producing biofuel and value-added products by cadmium tolerant microalgae as sustainable biorefinery approach”, Chem. Eng. Journal., 454, 140068, 2023
- Choudhary S, Tripathi S, Poluri KM* “Microalgal Based Bioenergy: Strategies, Prospects and Sustainability” ACS Energy & Fuels, 36 (24), 14584-14612, 2022
- Tripathi S, Behera T, Poluri KM* “Biochemical insights into cadmium detoxification mechanism of Coccomyxa sp. IITRSTKM4” J Environ Chem Eng., 10, 108102, 2022
- Sartaj Km, Gupta P, Tripathi S, Poluri KM, Prasad R “Insights into the extraction, characterization and antifungal activity of astaxanthin derived from yeast de-oiled biomass” Environmental Technology & Innovation, 27, 102437, 2022
- Tripathi S, Choudhary S, Poluri KM* “Insights into lipid accumulation features of Coccomyxa sp. IITRSTKM4 under nutrient limitation regimes” Environmental Technology & Innovation, 24, 101786, 2021
- Tripathi S, and Poluri KM* “Heavy metal detoxification mechanisms by microalgae: Insights from transcriptomics analysis” Environmental Pollution, 285, 117443, 2021
- Tripathi S, Arora N, Pruthi V, Poluri KM* “Elucidating the bioremediation mechanism of Scenedesmus sp. IITRIND2 under cadmium stress” Chemosphere, 283, 131196, 2021
- Joshi K#, Meher MK#, Poluri KM* “Fabrication and characterization of bioblocks from agricultural waste using fungal mycelium for renewable and sustainable applications”, ACS Applied Bio Materials., 3(4), 1884-92, 2020. [#Authors contributed equally]
- Batnagar VS, Bandyopadhyay P, Rajacharya GH, Sarkar S, Poluri KM, Kumar S “Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica”, Biotechnology for Biofuels, 12;176, 1-19, 2019.
- Arora N, Patel A, Mehtani J, Pruthi PA, Pruthi V*, Poluri KM* “Co-culturing of oleaginous microalgae and yeast: Paradigm shift towards enhanced lipid productivity”, Environ Sci Pollut Res. 26(17), 16952-73, 2019.
- Arora N, Laurens L, Sweeney N, Pruthi V, Poluri KM, Pienkos PT “Elucidating the unique physiological responses of halotolerant Scenedesmus sp. cultivated in sea water for biofuel production”, Algal Research, 37, 260-268, 2019.
- Arora N, Kumari P, Kumar A, Gangwar R, Gulati K, Pruthi PA, Prasad R, Kumar D*, Pruthi V*, Poluri KM* “Delineating the molecular responses of a halotolerant microalga using integrated omics approach to identify genetic engineering targets for enhanced TAG production”, Biotechnology for Biofuels, 12;2, 1-17, 2019.
- Arora N, Pienkos PT, Pruthi V, Poluri KM, Guarnieri M “Leveraging algal omics to reveal potential targets for augmenting TAG accumulation”, Biotechnology Advances 36, 1274-92, 2018.
- Arora N#, Dubey D#, Sharma M, Patel A, Guleria A, Pruthi PA, Kumar D*, Pruthi V*, Poluri KM* “NMR based metabolomics approach to elucidate the differential cellular responses during mitigation of arsenic (III, V) in a green microalga”, ACS Omega 3(9), 11847-56, 2018. (# Equal Contribution).
- Arora N, Patel A, Sharma M, Mehtani J, Pruthi PA, Pruthi V*, Poluri KM* “Insights into the enhanced lipid production characteristics of a fresh water microalga under high salinity conditions”, ACS Ind. Eng. Chem. Res. 56(25), 7413-21, 2017.
- Arora N, Gulati K, Patel A, Pruthi PA, Poluri KM*, Pruthi V* “A hybrid approach integrating arsenic detoxification with biodiesel production using oleaginous microalgae”, Algal Research 24, 29-39, 2017.
Honors And Awards
Science and Engineering Research Board
2022
SERB-STAR
Indian Institute of Technology Roorkee
2021
Ashok Soota Institute Research Fellow
Indian Institute of Technology Roorkee
2019
Institute Research Fellow
Department of Biotechnology
2013
Innovative Young Biotechnologist Award
National Academy of Sciences India
2014
NASI-Young Scientist Plantinum Jubilee Award
Andhra Pradesh Science City/AP Science Congress
2019
Prof. L. R. Row Memorial Award
Indian Science Congress Association
2009
Young Scientist Award
Indian Biophysical Society
2009
Best Oral Presentation
European Science Foundation
2007
International Student Fellowship
European Molecular Biology organization
2007
Visiting Scholar Fellowship
Royal Society of Chemistry, West India
2006
Best Oral Presentation
Nuclear Magnetic Resonance Society, India
2007
Best Poster Award
Acharya Nagarjuna University
2002
Gold Medal in M.Sc
Students
Delineating the Molecular Interactions of GRO Chemokines
15 Jul 2013 - 13 Nov 2017
Other Supervisors: , Scholar: Dr. Khushboo Gulati
Biophysical Studies on Structural Transitions of T7 Bacteriophage Endolysin
15 Jul 2013 - 22 Feb 2018
Other Supervisors: , Scholar: Dr. Meenakshi Sharma
Dissecting the molecular interactions of CCL2 chemokine with flavonoids
01 Jul 2014 - 26 Oct 2020
Other Supervisors: , Scholar: Dr. Nidhi Joshi
Evolution-structure-stability-function paradigm of CXC chemokine interleukin-8
22 Dec 2014 - 26 Oct 2020
Other Supervisors: , Scholar: Dr. Krishnakant Gangele
Elucidating the structural features and molecular interactions of histone like DNA binding protein (Hup) from H. pylori
26 Dec 2016 - 27 Jun 2022
Other Supervisors: , Scholar: Dr. Nipanshu Agarwal
Biochemical and metabolomic profiling of Candida glabrata isolates for pharmacological targeting
10 Jul 2019 - 13 Oct 2022
Other Supervisors: , Scholar: Dr. Hrishikesh Nandkishor Gupta
Delineating the structure-activity relationship of T7 bacteriophage endolysin
26 Dec 2016 - 28 Oct 2022
Other Supervisors: , Scholar: Dr. Jaya Lakshmi
Heparin based Nanocomposites for Biomedical applications
10 Jul 2017 - 13 Mar 2023
Other Supervisors: , Scholar: Dr. Mukesh Kumar Meher
Integrated Omics-Based Molecular Mapping of Cadmium Tolerant Microalga to Address Environment-Energy Nexus
10 Jul 2017 - 26 Jun 2023
Other Supervisors: , Scholar: Dr. Shweta Tripathi
Molecular Analysis of Chemokine CCL2-Inhibitor Interactions for Regulating Monocyte Traffickinge
10 Jul 2017 - 03 Jul 2024
Other Supervisors: , Scholar: Dr. Deepak Kumar Tripathi
Structural and Metabolomic Analysis of Bacteriphage Endolysins and Their Impact on Host Bacteria
26 Dec 2017 - 03 Jul 2024
Other Supervisors: , Scholar: Dr. Manikyaprabhu Kairamkonda
Functionalized Carageenan Based Nanomaterials For Mitigation of Hazardous Environmental Pollutants
10 Jul 2018 - 05 Jul 2024
Other Supervisors: , Scholar: Dr. Khushboo Rani Singh
Microalgae based Bioremediation & Biofuels
01 Jan 2019 - Present
Other Supervisors: , Scholar: Sonia Choudhary
Molecular Analysis of Chemokine Proteins
15 Jul 2019 - Present
Other Supervisors: , Scholar: Nupur Nagar
Molecular Characteristics of Bacteriophage Endolysins
15 Jul 2019 - Present
Other Supervisors: , Scholar: Mayur Mohan Ghate
Protein Biophysics
01 Feb 2021 - Present
Other Supervisors: , Scholar: Goutami Naidu
Protein nanocomposites for biotechnological applications
01 Aug 2021 - Present
Other Supervisors: , Scholar: Jinan Alhariry