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Prabhat Kumar Mandal
Associate Professor
mandal.prabhat[at]gmail.com
Dept of Biosciences and Bioengineering
91-1332-284780
Research Interests

Biosketch
Educational Details
Professional Background

Research
Projects
Publications
Patents
Books
Collaborations

Honours and Awards
Honors
Memberships

Teaching Engagements
Teaching Engagements

Students
Supervisions
Associate Scholars

Miscellaneous
Events
Visits
Administrative Positions
Miscellaneous
Research Interests
Cancer Biology, L1 retrotransposon activity in oral cancer, Human Genetics, Jumping genes in health and disease, Diagnosis, Generation of inhouse polyclonal and monoclonal antibody, cancer diagnosis kit
BioSketch
Educational Details
Calcutta University, West Bengal, India
1997
B.Sc, Physiology
Calcutta University, West Bengal, India
1999
M.Sc., Biophysics and Molecular Biology
Jawaharlal Nehru University, New Delhi, India
2006
Ph.D., Molecular Parasitology
Dept. of Genetics,University of Pennsylvania (USA)
2010
Postdoctoral Fellow, Human Genetics
Johns Hopkins University (USA)
2013
Postdoctoral Fellow, Human Genetics
Research
Projects
TOPIC START DATE FIELD DESCRIPTION FINANCIAL OUTLAY FUNDING AGENCY OTHER OFFICERS
Biology of Human LINE1 retrotransposon 05 Mar 2014 Biology of Human LINE1 retrotransposon 10 lacs FIG, IIT Roorkee
Identification of cellular factors with a role in human LINE1 retrotransposition 01 Nov 2015 Identification of cellular factors with a role in human LINE1 retrotransposition 47 lacs DST SERB
Investigating the mechanism of L1 retrotransposon activation in oral squamous cell carcinoma (52.36 lakhs , DST) 18 Feb 2022 Retrotransposon activation in cancer 50.82 Lacs DST Core Research Grant ( 50.82 Lachs)
Understanding the retrotransposon machinery through the delineation of LINE1-RNP complex composition in mice germ tissue 21 Dec 2022 Retrotransposon Biology 48.87 lacs DBT Basic Research (48.87 lacs)
Development of a membrane-based kit to find out L1 retrotransposon activity in Oral Squamous Cell Carcinoma (OSCC) 04 Feb 2022 Cancer and retrotransposons 11.23 lacs Uttarakhand State Council for Science and Technology (UCOST) Dr. Vandana Dhingra ( AIIMSR)
Investigations on selective proliferation and transport of Antibiotic Resistant Bacteria (ARBs) by microplastics (Co-PI) 19 Dec 2022 Antibiotic resistant bacteria 60 lacs ICMR Dr. Khalid M Gani , NIT Srinagar (PI-1), Dr. S Sarkar, Civil, IITR (PI-2)




Collaborations
Detection of Biomarkers and actionable target in Oral Squamous Cell Carcinoma (OSCC)
Prof. S P Agarwal, HOD, Dept. of Surgical Oncology, AIIMS, Rishikesh, India
Investigating L1 retrotransposon activity in the Human brain
Prof. Anita Mahadevan, Dept. of Neuropathology, NIMHANS, Bangalore, India
Biology of Human L1 retrotransposon
Prof. Haig Kazazian, Johns Hopkins University, USA
Investigation of L1 retrotransposon activity in Entamoeba histolytica
Prof. Sudha Bhattacharya, SES. Jawaharlal Nehru University New Delhi, India
Books

1. H.H. Kazazian, Jr., M.d.c. Seleme, D.V.Babushok, D.M. Ostertag, M.R. Vetter, P.K. Mandal. Human diversity and L1 Retrotransposons Biology: Creation of New Genes and Individual Variation in Retrotransposition Potential. Retrotransposition, Diversity and the Brain. Book Chapter, Yera 2008,Pages 43-52.

 

Patents

1. Title: Polyclonal human LINE-1 ORF2p antibody and method thereof. Patent no. 428643. Date of Filing 15.07.2019. Date of Grant 10.04.2023. Prabhat K Mandal, Koel Mukherjee, Debpali Sur.

2. Title: Process for the purification of polyclonal antibodies and applications thereof. Application No. 202411052502. Date of filing 09/07/2024. Prabhat K Mandal, Manali Gungali, Sandhya Rai, Sujoy Kundu, Gopal Sarkar and Suman Singh.

3. Title: Polyclonal antibody against L1ORF2p antigen and a method of preparing the same thereof. Application No.  202411062827. Date of filing 20.08.2024. Prabhat K Mandal, Gopal Sarkar and Sujoy Kundu.

 

4. Title: Affinity column purified polyclonal mouse L1ORF1p antibody and method thereof. Application No. 202511030130. Date of filing 28.05.2025. Prabhat K Mandal, Manali Gungali and Sujoy Kundu. 

 

Teaching Engagements
Teaching Engagements
Introduction to Biotechnology ( BTN-101 )
Autumn
Genetics ( BTN-505 )
Autumn
Genetic Engineering ( BTN-202 )
Spring
Biotechnology Lab 1 ( BTN-208 )
Spring
Students
SuperVisions
Investigating Human L1 Retrotransposon Activity in Oral Squamous Cell Carcinoma Patients
01 Jan 2013 - 29 May 2018
Other Supervisors: Prof. R Prasad, Scholar: Savita Budania
Studies on Human LINE-1 Retrotransposon Encoded ORF2 Protein
01 Jan 2014 - 11 Nov 2019
Other Supervisors: Prof. V Pruthi, Scholar: Sofia Pilli
Investigating Human Long Interspersed Element 1 (LINE 1) Activity in Health and Diseases
01 Jan 2015 - 09 Dec 2019
Other Supervisors: , Scholar: Debpali Sur
Municipal wastewater treatment plants as a reservoir of antibiotic resistance
01 Jan 2015 - 26 Nov 2021
Other Supervisors: Dr. Sudipta Sarkar (Civil Engineering), Scholar: Jahnavi K
Analysis of Tissue specific L 1 retrotransposon expression and L1 RNP characterization
01 Jan 2016 - Present
Other Supervisors: , Scholar: Koel Mukherjee
Biology of Human LINE1 retrotransposons
07 Aug 2020 - 06 Aug 2025
Other Supervisors: , Scholar: Mr. Sujoy Kundu
Membrane based kit to detect L1 retrotransposon activity in oral cancer
24 Jul 2021 - Present
Other Supervisors: , Scholar: Sandhya Rai
Investigating L-1 retrotransposon encoded ORF1 protein expression as a potential biomarker in OSCC
04 Aug 2018 - 07 Jun 2020
Other Supervisors: Prof. Satya Prakash Agarwal, Head, Dept. of Surgical Oncology, AIIMS Rishikesh, Scholar: Dr. ABHIJEET SINGH (MS, DNB) ( Surgical Oncology, AIIMS Rishikesh)
A study to identify new prognostic and predictive biomarkers in oral cancer patients with early recurrence
01 Jan 2020 - Present
Other Supervisors: Dr. KS Rajkumar, Dept. of Head Neck Surgical Oncology, AIIMS Rishikesh, India, Scholar: Dr. Shahab Ali Usmani ( Surgical Oncology, AIIMS Rishikesh)
Miscellaneous
Events
Jumping genes become active in critical brain areas as we age
01 Jan 2018 - Present
Role of mobile genetic elements in human health and disease
01 Jan 2020 - Present
L1 retrotrasposon activity in normal human brain and in oral cancer
01 Jan 2020 - Present
Extensive LINE-1 promoter hypomethylation and ORF1p expression in oral squamous cell carcinoma
01 Jan 2018 - Present
The influence of age on jumping gene activity in human brain.
01 Jan 2017 - Present
L1 retrotransposon activity in cancer.
01 Jan 2016 - Present
Administrative Positions
OC Time Table
01 Jan 2014 - 01 Jan 2017
IIT Roorkee
OC Dept. Animal Facility
01 Jan 2017 - Present
IIT Roorkee
OC Dept. Central Lab 112
01 Jan 2017 - Present
IIT Roorkee
Member, Dept. Wast Management
01 Jan 2017 - Present
IIT Roorkee
Member, DRC
06 Aug 2023 - Present
IIT Roorkee
OC Dept. Radioactive Facility
01 Jan 2017 - Present
IIT Roorkee
Referred Journal papers

2021

20. Kurasam J, Mandal PK, Sarkar S. Selective Proliferation of Antibiotic-Resistant Bacteria in the Biological Treatment Process at a Municipal Wastewater Treatment Plant in India. Journal of Environmental Engineering (ASCE) (Accepted Nov 2021, Equb ahead of print)

19. Mukherjee KSur D, Sing A, Rai S, Das N, Sekar R, Narindi S, Dhingra V, Jat B, Balraam, V, Agarwal S, Mandal PK*. Robust expression of LINE-1 retrotransposon encoded proteins in oral squamous cell carcinoma. BMC cancer (Nature Springer) 2021, accepted. (Impact Factor 3.2). 

18. Mukherjee KSur D, Sing A, Rai S, Das N, Sekar R, Narindi S, Dhingra V, Jat B, Balraam, V, Agarwal S, Mandal PK*. Robust expression of LINE-1 retrotransposon encoded proteins in oral squamous cell carcinoma. biorxiv.org doi:https://doi.org/10.1101/2020.11.24.395285. 

17. Kaur D, Agrahari M, Singh S, Mandal PK, Bhattacharya A, Bhattacharya S*. Transcriptomic analysis of Entamoeba histolytica reveals domain-specific sense strand expression of LINE-encoded ORFs with massive antisense expression of RT domain. Plasmid (Elsevier),2021,114,102560. doi: 10.1016/j.plasmid.2021. (Impact Factor 2.85). 

2020

16.  Budania S, Sur D, Nangal J, Pilli S, Mukherjee K, Biswas M, Prasad R, Saxena S, and Mandal PK*. LINE-1 retrotransposon encoded ORF1p expression and promoter methylation in oral squamous cell carcinoma. Cancer Genetics (Elsevier), 2020, 244: 21-29. (*Corresponding author). (Impact Factor-3.1). 

2018

15. Kurasam J, Sihag P, Mandal PK, Sarkar S.  Presence of Fluoroquinolone Resistance with Persistent Occurrence of GyrA Gene Mutations in a Municipal Wastewater Treatment Plant in India. Chemosphere. 2018, 211: 817-825. DOI: 10.1016/j..2018.08.011 (Impact Factor-4.42). 

2017

14. Singh B, Verma A, Pooja, Mandal PK, Datta S. A biotechnological approach for degradation of inhibitory compounds present in lignocellulosic biomass hydrolysate liquor using Bordetella sp. BTIITR. Chemical Engineering Journal, 2017, 328, 519–526. DOI: 10.1016/j.cej.2017.07.059 (Impact Factor-10.6). 

13. Kumara R, Anjlika, Ratnam A, Kumar S, Bala M, Sur D, Narang S, Singh UP, Mandal PK*, Ghosh S*. Organometallic ruthenium nitrosyl obtained by C-H bond activation: Photo-induced delivery of nitric oxide (NO) and NO mediated anti-proliferation activity studies.  European Journal of Inorganic Chemistry. 2017, 5334-5343.DOI:10.1002/ejic.201700839 (Impact Factor-2.5). 

12. Sur D, Kustwar RK, Budania S, Mahadevan S, Hancks DC, Yadav V, Shankar SK and Mandal PK*. Detection of the LINE-1 retrotransposon RNA-binding protein ORF1p in different anatomical regions of the human brain. BMC Mobile DNA (Nature Springer). 2017; 8:17. doi: 10.1186/s13100-017-0101-4  (Impact Factor-5.1). 

2016

11. Mandal PK and Kazazian HH. Purification of L1-ribonucleoprotein particles (L1-RNPs) from cultured human cells. Methods in Molecular Biology (Springer) on "Retrotransposons and Transposons, 2016.1400, 299-310. 

Post Doc Publications: 

10. Mandal PK, Ewing AE, Hancks DC, Kazazian HH Jr.  Enrichment of processed pseudogene transcripts in L1- ribonucleoprotein particles. Human Molecular Genetics. 2013. 22. 3730-3748. 

9. Hancks DC, Mandal PK, Cheung LE, Kazazian HH Jr. The minimal active human SVA retrotransposon requires Only the 5'-hexamer and Alu-like domains. Molecular and Cellular Biology. 2012.32:4718-4726. 

8. Hancks DC, Goodier JL*, Mandal PK*, Cheung LE, Kazazian HH Jr. Retrotransposition of marked SVA elements by human L1s in cultured cells. Human Molecular Genetics. 2011.20:3386-3400. (*Equal Contribution). 

7. Goodier JL, Mandal PK, Zhang L, Kazazian HH Jr. Discrete subcellular partitioning of human retrotransposon RNAs despite a common mechanism of genome insertion. Human Molecular Genetics. 2010.19: 1712-1725. 

6. Mandal PK, Kazazian HH Jr. Snapshot: Vertebrate Transposons. Cell. 2008.135: 192.  

5. Babushok DV, Ohshima K, Ostertag EM, Chen X, Wang Y, Mandal PK, Okada N, Abrams CS,  Kazazian HH Jr, A novel testis ubiquitin binding protein, S5a-like, arose by exon-shuffling in hominoids. Genome Research. 2007.17: 1129-1138. 

Ph.D Publications: 

4. Yadav VP, Mandal PK, Bhattacharya A, Bhattacharya S. SINE chimeras are formed at high frequency during induced retrotransposition in vivo. Nature communications. 2012 DOI:10.1038. 

3. Yadav VP, Mandal PK, Rao DN, Bhattacharya S. Characterization of the restriction enzyme-like endonuclease encoded by the Entamoeba histolytica non-long terminal repeat retrotransposon EhLINE1. FEBS J. 2009, 276:7070-7082. 

2. Mandal PK, Rawal K, Ramaswamy R, Bhattacharya A, Bhattacharya S. Identification of insertion hot spots for non-LTR retrotransposons: computational and biochemical application to Entamoeba histolytica. Nucleic Acids Res. 2006, 34(20): 5752-5763. 

1. Mandal PK, Bagchi A, Bhattacharya A, Bhattacharya S. An Entamoeba histolytica LINE/SINE pair inserts at common target sites cleaved by the restriction enzyme-like LINE-encoded endonuclease. Eukaryotic Cell. 2004, 3(1): 170-179.