Prabhat Kumar Mandal

1. Title: Polyclonal human LINE-1 ORF2p antibody and method thereof. Patent no. 428643; Date of Filing 15.07.2019. Date of Grant 10.04.2023; Prabhat K Mandal, Koel Mukherjee, Debpali Sur.

2. Title: Process for the purification of polyclonal antibodies and applications thereof. Patent No. 576286; Date of filing 09.07.2024; Date of Grant 22. 12.2025; Prabhat K Mandal, Manali Ganguly, Sandhya Rai, Sujoy Kundu, Gopal Sarkar and Suman Singh.

3. Title: Cloned vector for polyclonal antibody against L1ORF2p and a method of preparing the same thereof. Patent No. 585660. Patentee: Mandal PK, Sarkar G and Kundu S. Date of Filing 20.08.2024. Date of Grant 30.03.2026.

4. Title: Affinity column purified polyclonal mouse L1ORF1p antibody and method thereof. Application No. 202511030130. Date of filing 28.05.2025. Prabhat K Mandal, Manali Gungali and Sujoy Kundu.

5. Title: Lateral flow immunoassay for detection of L1ORF1p biomarker. Application No. 202611044511. Date of filing 07.04.2026. Mandal PK, Rai S.

20. Gopal Sarkar, Sujoy Kundu, Sulakshana P Mukherjee, John L Goodier and Prabhat K Mandal^*. Detection of endogenous LINE-1 ORF2p and its potent reverse transcriptase activity in the MCF-7 breast cancer cell line. The FEBS Journal (FEBS Press, Wiley); 2025; doi:10.1111/febs.70375.

19. Kurasam J, Mandal PK, Sarkar S. Selective Proliferation of Antibiotic-Resistant Bacteria in the Biological Treatment Process at a Municipal Wastewater Treatment Plant in India. Journal of Environmental Engineering (ASCE) 148 (4), 2022; DOI: 10.1061/(ASCE)EE.1943-7870.0001980. (Impact Factor 1.85).

18. Mukherjee K, Sur D, Sing A, Rai S, Das N, Sekar R, Narindi S, Dhingra V, Jat B, Balraam, V, Agarwal S, Mandal PK*. Robust expression of LINE-1 retrotransposon encoded proteins in oral squamous cell carcinoma. BMC cancer (Nature Springer) 2021.

17. Kaur D, Agrahari M, Singh S, Mandal PK, Bhattacharya A, Bhattacharya S*. Transcriptomic analysis of Entamoeba histolytica reveals domain-specific sense strand expression of LINE-encoded ORFs with massive antisense expression of RT domain. Plasmid (Elsevier),2021,114,102560. doi: 10.1016/j.plasmid.2021

16. Budania S, Sur D, Nangal J, Pilli S, Mukherjee K, Biswas M, Prasad R, Saxena S, and Mandal PK^*. LINE-1 retrotransposon encoded ORF1p expression and promoter methylation in oral squamous cell carcinoma. Cancer Genetics (Elsevier), 2020, 244: 21-29.

15. Kurasam J, Sihag P, Mandal PK, Sarkar S. Presence of Fluoroquinolone Resistance with Persistent Occurrence of GyrA Gene Mutations in a Municipal Wastewater Treatment Plant in India. Chemosphere. 2018, 211: 817-825. DOI: 10.1016/j..2018.08.011.

14. Singh B, Verma A, Pooja, Mandal PK, Datta S. A biotechnological approach for degradation of inhibitory compounds present in lignocellulosic biomass hydrolysate liquor using Bordetella sp. BTIITR. Chemical Engineering Journal, 2017, 328, 519–526. DOI: 10.1016/j.cej.2017.07.059.

13. Kumara R, Anjlika, Ratnam A, Kumar S, Bala M, Sur D, Narang S, Singh UP, Mandal PK*, Ghosh S*. Organometallic ruthenium nitrosyl obtained by C-H bond activation: Photo-induced delivery of nitric oxide (NO) and NO mediated anti-proliferation activity studies. European Journal of Inorganic Chemistry. 2017, 5334-5343.DOI:10.1002/ejic.201700839.

12. Sur D, Kustwar RK, Budania S, Mahadevan S, Hancks DC, Yadav V, Shankar SK and Mandal PK^*. Detection of the LINE-1 retrotransposon RNA-binding protein ORF1p in different anatomical regions of the human brain. BMC Mobile DNA (Nature Springer). 2017; 8:17. doi: 10.1186/s13100-017-0101-4 (Impact Factor-5.1).

11. Mandal PK and Kazazian HH. Purification of L1-ribonucleoprotein particles (L1-RNPs) from cultured human cells. Methods in Molecular Biology (Springer) on "Retrotransposons and Transposons, 2016.1400, 299-310.

Post Doc Publications:

10. Mandal PK, Ewing AE, Hancks DC, Kazazian HH Jr. Enrichment of processed pseudogene transcripts in L1- ribonucleoprotein particles. Human Molecular Genetics. 2013. 22. 3730-3748.

9. Hancks DC, Mandal PK, Cheung LE, Kazazian HH Jr. The minimal active human SVA retrotransposon requires Only the 5'-hexamer and Alu-like domains. Molecular and Cellular Biology. 2012.32:4718-4726.

8. Hancks DC, Goodier JL*, Mandal PK*, Cheung LE, Kazazian HH Jr. Retrotransposition of marked SVA elements by human L1s in cultured cells. Human Molecular Genetics. 2011.20:3386-3400. (*Equal Contribution).

7. Goodier JL, Mandal PK, Zhang L, Kazazian HH Jr. Discrete subcellular partitioning of human retrotransposon RNAs despite a common mechanism of genome insertion. Human Molecular Genetics. 2010.19: 1712-1725.

6. Mandal PK, Kazazian HH Jr. Snapshot: Vertebrate Transposons. Cell. 2008.135: 192.

5. Babushok DV, Ohshima K, Ostertag EM, Chen X, Wang Y, Mandal PK, Okada N, Abrams CS, Kazazian HH Jr, A novel testis ubiquitin binding protein, S5a-like, arose by exon-shuffling in hominoids. Genome Research. 2007.17: 1129-1138.

Ph.D Publications:

4. Yadav VP, Mandal PK, Bhattacharya A, Bhattacharya S. SINE chimeras are formed at high frequency during induced retrotransposition in vivo. Nature communications. 2012 DOI:10.1038.

3. Yadav VP, Mandal PK, Rao DN, Bhattacharya S. Characterization of the restriction enzyme-like endonuclease encoded by the Entamoeba histolytica non-long terminal repeat retrotransposon EhLINE1. FEBS J. 2009, 276:7070-7082.

2. Mandal PK, Rawal K, Ramaswamy R, Bhattacharya A, Bhattacharya S. Identification of insertion hot spots for non-LTR retrotransposons: computational and biochemical application to Entamoeba histolytica. Nucleic Acids Res. 2006, 34(20): 5752-5763.

1. Mandal PK, Bagchi A, Bhattacharya A, Bhattacharya S. An Entamoeba histolytica LINE/SINE pair inserts at common target sites cleaved by the restriction enzyme-like LINE-encoded endonuclease. Eukaryotic Cell. 2004, 3(1): 170-179.

TOPIC	START DATE	FIELD	DESCRIPTION	FINANCIAL OUTLAY	FUNDING AGENCY	OTHER OFFICERS
Biology of Human LINE1 retrotransposon	05 Mar 2014	Biology of Human LINE1 retrotransposon		10 lacs	FIG, IIT Roorkee
Identification of cellular factors with a role in human LINE1 retrotransposition	01 Nov 2015	Identification of cellular factors with a role in human LINE1 retrotransposition		47 lacs	DST SERB
Investigating the mechanism of L1 retrotransposon activation in oral squamous cell carcinoma (52.36 lakhs , DST)	18 Feb 2022	Retrotransposon activation in cancer		50.82 Lacs	DST Core Research Grant ( 50.82 Lachs)
Understanding the retrotransposon machinery through the delineation of LINE1-RNP complex composition in mice germ tissue	21 Dec 2022	Retrotransposon Biology		48.87 lacs	DBT Basic Research (48.87 lacs)
Development of a membrane-based kit to find out L1 retrotransposon activity in Oral Squamous Cell Carcinoma (OSCC)	04 Feb 2022	Cancer and retrotransposons		11.23 lacs	Uttarakhand State Council for Science and Technology (UCOST)	Dr. Vandana Dhingra ( AIIMSR)
Investigations on selective proliferation and transport of Antibiotic Resistant Bacteria (ARBs) by microplastics (Co-PI)	19 Dec 2022	Antibiotic resistant bacteria		60 lacs	ICMR	Dr. Khalid M Gani , NIT Srinagar (PI-1), Dr. S Sarkar, Civil, IITR (PI-2)