Research Interests
Cancer Biology, L1 retrotransposon activity in oral cancer, Human Genetics, Jumping genes in health and disease, Diagnosis, Generation of inhouse polyclonal and monoclonal antibody, cancer diagnosis kit
BioSketch
Research
1. Title: Polyclonal human LINE-1 ORF2p antibody and method thereof. Patent no. 428643. Date of Filing 15.07.2019. Date of Grant 10.04.2023. Prabhat K Mandal, Koel Mukherjee, Debpali Sur.
2. Title: Process for the purification of polyclonal antibodies and applications thereof. Application No. 202411052502. Date of filing 09/07/2024. Prabhat K Mandal, Manali Gungali, Sandhya Rai, Sujoy Kundu, Gopal Sarkar and Suman Singh.
3. Title: Polyclonal antibody against L1ORF2p antigen and a method of preparing the same thereof. Application No. 202411062827. Date of filing 20.08.2024. Prabhat K Mandal, Gopal Sarkar and Sujoy Kundu.
4. Title: Affinity column purified polyclonal mouse L1ORF1p antibody and method thereof. Application No. 202511030130. Date of filing 28.05.2025. Prabhat K Mandal, Manali Gungali and Sujoy Kundu.
Teaching Engagements
Students
Miscellaneous
2021
20. Kurasam J, Mandal PK, Sarkar S. Selective Proliferation of Antibiotic-Resistant Bacteria in the Biological Treatment Process at a Municipal Wastewater Treatment Plant in India. Journal of Environmental Engineering (ASCE) (Accepted Nov 2021, Equb ahead of print)
19. Mukherjee K, Sur D, Sing A, Rai S, Das N, Sekar R, Narindi S, Dhingra V, Jat B, Balraam, V, Agarwal S, Mandal PK*. Robust expression of LINE-1 retrotransposon encoded proteins in oral squamous cell carcinoma. BMC cancer (Nature Springer) 2021, accepted. (Impact Factor 3.2).
18. Mukherjee K, Sur D, Sing A, Rai S, Das N, Sekar R, Narindi S, Dhingra V, Jat B, Balraam, V, Agarwal S, Mandal PK*. Robust expression of LINE-1 retrotransposon encoded proteins in oral squamous cell carcinoma. biorxiv.org doi: https://doi.org/10.1101/2020.11.24.395285.
17. Kaur D, Agrahari M, Singh S, Mandal PK, Bhattacharya A, Bhattacharya S*. Transcriptomic analysis of Entamoeba histolytica reveals domain-specific sense strand expression of LINE-encoded ORFs with massive antisense expression of RT domain. Plasmid (Elsevier),2021,114,102560. doi: 10.1016/j.plasmid.2021. (Impact Factor 2.85).
2020
16. Budania S, Sur D, Nangal J, Pilli S, Mukherjee K, Biswas M, Prasad R, Saxena S, and Mandal PK*. LINE-1 retrotransposon encoded ORF1p expression and promoter methylation in oral squamous cell carcinoma. Cancer Genetics (Elsevier), 2020, 244: 21-29. (*Corresponding author). (Impact Factor-3.1).
2018
15. Kurasam J, Sihag P, Mandal PK, Sarkar S. Presence of Fluoroquinolone Resistance with Persistent Occurrence of GyrA Gene Mutations in a Municipal Wastewater Treatment Plant in India. Chemosphere. 2018, 211: 817-825. DOI: 10.1016/j..2018.08.011 (Impact Factor-4.42).
2017
14. Singh B, Verma A, Pooja, Mandal PK, Datta S. A biotechnological approach for degradation of inhibitory compounds present in lignocellulosic biomass hydrolysate liquor using Bordetella sp. BTIITR. Chemical Engineering Journal, 2017, 328, 519–526. DOI: 10.1016/j.cej.2017.07.059 (Impact Factor-10.6).
13. Kumara R, Anjlika, Ratnam A, Kumar S, Bala M, Sur D, Narang S, Singh UP, Mandal PK*, Ghosh S*. Organometallic ruthenium nitrosyl obtained by C-H bond activation: Photo-induced delivery of nitric oxide (NO) and NO mediated anti-proliferation activity studies. European Journal of Inorganic Chemistry. 2017, 5334-5343.DOI:10.1002/ejic.201700839 (Impact Factor-2.5).
12. Sur D, Kustwar RK, Budania S, Mahadevan S, Hancks DC, Yadav V, Shankar SK and Mandal PK*. Detection of the LINE-1 retrotransposon RNA-binding protein ORF1p in different anatomical regions of the human brain. BMC Mobile DNA (Nature Springer). 2017; 8:17. doi: 10.1186/s13100-017-0101-4 (Impact Factor-5.1).
2016
11. Mandal PK and Kazazian HH. Purification of L1-ribonucleoprotein particles (L1-RNPs) from cultured human cells. Methods in Molecular Biology (Springer) on "Retrotransposons and Transposons, 2016.1400, 299-310.
Post Doc Publications:
10. Mandal PK, Ewing AE, Hancks DC, Kazazian HH Jr. Enrichment of processed pseudogene transcripts in L1- ribonucleoprotein particles. Human Molecular Genetics. 2013. 22. 3730-3748.
9. Hancks DC, Mandal PK, Cheung LE, Kazazian HH Jr. The minimal active human SVA retrotransposon requires Only the 5'-hexamer and Alu-like domains. Molecular and Cellular Biology. 2012.32:4718-4726.
8. Hancks DC, Goodier JL*, Mandal PK*, Cheung LE, Kazazian HH Jr. Retrotransposition of marked SVA elements by human L1s in cultured cells. Human Molecular Genetics. 2011.20:3386-3400. (*Equal Contribution).
7. Goodier JL, Mandal PK, Zhang L, Kazazian HH Jr. Discrete subcellular partitioning of human retrotransposon RNAs despite a common mechanism of genome insertion. Human Molecular Genetics. 2010.19: 1712-1725.
6. Mandal PK, Kazazian HH Jr. Snapshot: Vertebrate Transposons. Cell. 2008.135: 192.
5. Babushok DV, Ohshima K, Ostertag EM, Chen X, Wang Y, Mandal PK, Okada N, Abrams CS, Kazazian HH Jr, A novel testis ubiquitin binding protein, S5a-like, arose by exon-shuffling in hominoids. Genome Research. 2007.17: 1129-1138.
Ph.D Publications:
4. Yadav VP, Mandal PK, Bhattacharya A, Bhattacharya S. SINE chimeras are formed at high frequency during induced retrotransposition in vivo. Nature communications. 2012 DOI:10.1038.
3. Yadav VP, Mandal PK, Rao DN, Bhattacharya S. Characterization of the restriction enzyme-like endonuclease encoded by the Entamoeba histolytica non-long terminal repeat retrotransposon EhLINE1. FEBS J. 2009, 276:7070-7082.
2. Mandal PK, Rawal K, Ramaswamy R, Bhattacharya A, Bhattacharya S. Identification of insertion hot spots for non-LTR retrotransposons: computational and biochemical application to Entamoeba histolytica. Nucleic Acids Res. 2006, 34(20): 5752-5763.
1. Mandal PK, Bagchi A, Bhattacharya A, Bhattacharya S. An Entamoeba histolytica LINE/SINE pair inserts at common target sites cleaved by the restriction enzyme-like LINE-encoded endonuclease. Eukaryotic Cell. 2004, 3(1): 170-179.