About Instrument and its Applications:
Gel permeation chromatography (GPC) is a type of size-exclusion chromatography (SEC), that separates analytes on the basis of size, typically in organic solvents. When characterizing polymers, it is important to consider the dispersity (Đ) as well the molecular weight. Polymers can be characterized by a variety of definitions for molecular weight including the number average molecular weight (Mn), the weight average molecular weight (Mw) (see molar mass distribution), the size average molecular weight (Mz). GPC allows for the determination of Đ, based on other data, the Mn, Mw, and Mz can be determined.
GPC separates based on the size or hydrodynamic volume (radius of gyration) of the analytes. This differs from other separation techniques which depend upon chemical or physical interactions to separate analytes. Separation occurs via the use of porous beads packed in a column.
The smaller analytes can enter the pores more easily and therefore spend more time in these pores, increasing their retention time. These smaller molecules spend more time in the column and therefore will elute last. Conversely, larger analytes spend little if any time in the pores and are eluted quickly. All columns have a range of molecular weights that can be separated.
General Information:
Make – WGE Dr. Bures
Model – SEC 3010
Year of Installation – 2014
Specification:
Suitable Column Solvent: THF only
Sample Requirements:
Sample should be dissolved properly in THF and a solution of 1 mg/ml concentration may be prepared.